Plasminogen Activators in Dextran Sulfate - Activated Euglobulin Fractions : A
نویسنده
چکیده
To elucidate the mechanism by which activation of the contact system of blood coagulation leads to expression of fibrinolytic activity, we have determined the molecular characteristics of the plasminogen activators present in dextran sulfate-treated euglobulin fractions by electrophoretic-zymographic analysis and specific immunoadsorption. In addition to free and protease inhibitor-bound tissue-type plasminogen activator (t-PA), dextran sulfate precipitates of euglobulins contained the complex formed between plasma kallikrein and Cl -inhibitor. an indicator of prekallikrein activation. These precipitates also contained sub-
منابع مشابه
Activators in Dextran Sulfate - Activated Euglobulin Fractions : A
To elucidate the mechanism by which activation of the contact system of blood coagulation leads to expression of fibrinolytic activity, we have determined the molecular characteristics of the plasminogen activators present in dextran sulfate-treated euglobulin fractions by electrophoretic-zymographic analysis and specific immunoadsorption. In addition to free and protease inhibitor-bound tissue...
متن کاملPlasminogen activators in dextran sulfate-activated euglobulin fractions: a molecular analysis of factor XII- and prekallikrein-dependent fibrinolysis.
To elucidate the mechanism by which activation of the contact system of blood coagulation leads to expression of fibrinolytic activity, we have determined the molecular characteristics of the plasminogen activators present in dextran sulfate-treated euglobulin fractions by electrophoretic-zymographic analysis and specific immunoadsorption. In addition to free and protease inhibitor-bound tissue...
متن کاملThe Effect of Dilution, pH and Ionic Strength of Plasma on t-PA Precipitation in Euglobulin Fraction
In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditions by changing pH, ionic strength and degree of dilution. The precipitation of t-PA Ag in the eug...
متن کاملEuglobulin lysis times: an update.
UNLABELLED There are several clot lysis tests available to assess the presence of increased fibrinolysis in a clinical situation. These include whole blood, plasma, and "eugloblin" lysis tests. The euglobulin lysis test (ELT) is actually a modified plasma clot lysis time, which detects increased plasminogen activation and subsequent fibrinolysis. It does not detect the activity of antiplasmins....
متن کاملThe Immunological Specificity of the Euglobu- Lin and Pseudoglobulin Fractions of Horse and Human Serum
Serum is generally considered to contain at least two proteins, albumin and globulin, differing both in their isoelectric points, (literature summarized by Freundlich) and molecular weights (Svedberg and Sj6gren), and readily separable by half-saturation with ammonium sulfate, which precipitates the globulin. There has been considerable investigation tending to show that the globulin fraction i...
متن کامل